Regulatory and Essential Light Chains of Myosin Rotate Equally during Contraction of Skeletal Muscle
نویسندگان
چکیده
منابع مشابه
Phosphorylation of the regulatory light chains of myosin affects Ca sensitivity of skeletal muscle contraction
Szczesna, Danuta, Jiaju Zhao, Michelle Jones, Gang Zhi, James Stull, and James D. Potter. Phosphorylation of the regulatory light chains of myosin affects Ca2 sensitivity of skeletal muscle contraction. J Appl Physiol 92: 1661–1670, 2002. First published December 21, 2001; 10.1152/japplphysiol.00858.2001.—The role of phosphorylation of the myosin regulatory light chains (RLC) is well establishe...
متن کاملPhosphorylation of the regulatory light chains of myosin affects Ca2+ sensitivity of skeletal muscle contraction.
The role of phosphorylation of the myosin regulatory light chains (RLC) is well established in smooth muscle contraction, but in striated (skeletal and cardiac) muscle its role is still controversial. We have studied the effects of RLC phosphorylation in reconstituted myosin and in skinned skeletal muscle fibers where Ca2+ sensitivity and the kinetics of steady-state force development were meas...
متن کاملMyosin light chain kinase and myosin phosphorylation effect frequency-dependent potentiation of skeletal muscle contraction.
Repetitive stimulation potentiates contractile tension of fast-twitch skeletal muscle. We examined the role of myosin regulatory light chain (RLC) phosphorylation in this physiological response by ablating Ca(2+)/calmodulin-dependent skeletal muscle myosin light chain kinase (MLCK) gene expression. Western blot and quantitative-PCR showed that MLCK is expressed predominantly in fast-twitch skel...
متن کاملAge- and Activity-Related Differences in the Abundance of Myosin Essential and Regulatory Light Chains in Human Muscle
Traditional methods for phenotyping skeletal muscle (e.g., immunohistochemistry) are labor-intensive and ill-suited to multixplex analysis, i.e., assays must be performed in a series. Addressing these concerns represents a largely unmet research need but more comprehensive parallel analysis of myofibrillar proteins could advance knowledge regarding age- and activity-dependent changes in human m...
متن کاملFunctional roles of essential light chains of myosin II
The C-terminus of myosin-S1 forms the alpha-helical lever arm which is associated with an essential and a regulatory light chain. The lever arm is believed to work as an elastic ratchet which moves during force-generation from an up into a down position. The ELC in the heart tethers the tick and thin filaments by binding with its four EF-hand domains to the myosin lever arm, while the antenna-l...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2002
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(02)75657-9